Abstract:
Dry biomass of algae is a good source of nutrients and biologically active substances, which in the recent years attracted the interest of the specialists in their search for natural, ecologically and healthy sound foods for the animals. The aim of the present study was to determine the mineral composition, the antioxidant and antimicrobial
activities of the dry biomass of freshwater algae (spirogyra genus). The freshwater algae were collected in February and March 2010 at the pond of Jomo Kenyatta University of Agriculture and Technology (JKUAT). The Mineral content by acid digestion (HNO3:HClO) in mg /100g was : 387.22 ± 38.72 Ca, 384.99 ± 39.17 Mg, 244.44±17.10 Fe, 2.53 ± 0.48 Cu, 7.25 ± 0.55 Zn, Phytochemical screening of methanolic and water extract showed the presence 4
of alkaloids, steroids, flavonoids, tannins, terpenoids, but only the water extract showed the presence of total reducing sugars. The content of phenolic compounds and tannins was 10.63 ± 0.05g gallic acid/100g of extract and 0.399 ± 0.02g tannic acid/100g of dried sample and the level of flavonoids was 6.86 ± 0.14g Quercetin/100g of extract. The algal antioxidant activity expressed in a dose able to decrease the initial DPPH concentration by 50%,
or IC was 0.078 mg/ml. The antimicrobial activity of methanolic extract was efficient against Escherichia coli and
Candida albicans. The water extract didn’t show any activity. The freshwater algae used in this study were identified as Spirogyra nitida associated Spirogyra weberi both filamentous green algae. The mineral composition showed that they contain minerals which give the possibility of using them as food for fish and also by human as food supplement. The levels of Ca, Mg and Fe were ver y high as the Spirulina and Chlorella are used as food supplement. The strains showed a relative antioxidant and antimicrobial activities.