Evaluation of In vivo and In vitro Antioxidant properties of the Ethanol Leaf Extract of Culcasia falcifolia

Abstract

Objective: To evaluate the in vivo antioxidant potential of the ethanol leaf extract of Culcasia falcifolia against Pentylenetetrazole induced oxidative stress as well as its in vitro antioxidant effect. Method: The extract was prepared by maceration using ethanol. Four groups of six mice were used for the in vivo studies. Group I (control group) received 0.1% CMC. Group II (positive control) received diazepam on the experimental day (5mg/kg body wt.). Group III and IV received 200 and 400 mg/kg body wt. of ethanol extract of C. falcifolia orally for fourteen days. On the fifteenth, all mice were sacrificed to remove the brains after the injection of PTZ (60 mg/kg i.p). MDA, SOD, CAT, GPX and GR levels were estimated from the brain tissue. DPPH radical scavenging assay and ferrous ion chelating assay were used to evaluate the in vitro studies. Result: The study suggests that the extract exhibit in vivo antioxidant property by increasing in the levels of SOD, CAT, GPX and GR and decreasing in the MDA level of the brain tissue significantly (p<0.05*). The in vitro studies also showed that the extract was able to scavenge free radical and chelate metal. Conclusion: The ethanol extract of Culcasia falcifolia possesses in vivo and in vitro antioxidant activity against Pentylentetrazole induced seizure in mice.